Does it matter what kind of protein I eat, or does only the total amount count?
The type of protein matters: animal proteins and well-combined plant-based sources provide more building material for muscles than collagen or single soy products. The total amount counts too, but it is not the whole story.
How many essential amino acids appear in your blood after a meal largely determines how much muscle tissue you build. Not the total grams of protein on the label, but the quantity and speed of those amino acids entering the bloodstream is what matters. A doubling of essential amino acids in the blood was associated with approximately 34% greater muscle protein synthesis, regardless of whether those amino acids came from free-form supplements, whey, or ordinary food.
Plant-based proteins such as soy and wheat score lower on this point than animal proteins. They are harder to digest, contain less leucine (the amino acid that most strongly stimulates muscle building), and provide relatively little lysine and methionine. You can partially compensate for this by eating more, combining plant-based sources, or supplementing them with specific amino acids. Note that this has been studied almost exclusively in soy and wheat. How other plant-based proteins, such as peas, rice, or lupin, behave is largely unknown.
Collagen deserves special mention. It counts on paper as protein, but it contains virtually no leucine and has an incomplete amino acid profile. Gram for gram, collagen provides far less stimulus for muscle building than whey or casein. For joints and connective tissue, 15 grams of collagen per day is worthwhile, but anyone looking to build muscle is better off getting the rest of their protein intake from higher-quality sources.
The total amount of protein also matters, but in a different way: during weight loss, consuming somewhat more protein than the standard guideline (more than 0.8 grams per kilogram of body weight per day) helps preserve muscle mass. This effect is particularly visible in older adults, postmenopausal women, and people with excess weight. The point here is not to eat as much as possible, but to eat enough. Dietary context also plays a role: in a ketogenic diet, where carbohydrates are severely restricted but protein intake remains the same, muscle growth lagged behind compared to a regular Western diet. This suggests that protein does not operate independently of everything else you eat.
All claims are based on human studies, mostly small to medium-sized trials and reviews. The association between blood amino acids and muscle protein synthesis is the most quantitatively well-supported finding. Results regarding plant-based proteins apply almost exclusively to soy and wheat. The keto study included only 19 participants over 2 months and is therefore too small to draw firm conclusions.